Figure 2
From: SILAC–based quantitative MS approach for real-time recording protein-mediated cell-cell interactions
Quantitative MS peaks of three proteins identified by spike-in SILAC and Triple-SILAC in mouse Ana-1 and CT26 co-culture system. In triple-SILAC way, the MS spectra showed the peak intensity of the quantitative peptides with none (L), 2H3-Leu (M) and 13C6-Lys (H) labels. In spike-in SILAC, the quantitative peptide containing 2H3-Leu (M) and 13C6-Lys (H) labels were compared to none labeling (L) separately. In both ways, the monoisotopic peaks with highest intensity were taken to be compared. (A) The MS spectra of the quantitative peptide“R.LNMEAINYMAADGDFK.I” for Galectin-1. (B)The quantitative peptide “K.ENGGLDSEESYPYEAK.D” for Cathepsin L1. (C) The quantitative peptide “K.AGTLDLSLSLPGK.Q” for Thrombospondin-1.
