Table 3 Advantages and disadvantages of two SILAC–based MS approaches.
From: SILAC–based quantitative MS approach for real-time recording protein-mediated cell-cell interactions
Name | Description | Advantages | Disadvantages |
|---|---|---|---|
spike-in SILAC | Decoupled mixtures of two kinds of labeling cells with a heavy/light spike-in standard | 1. Tissues applicable 2. High coverage 3. Multiple samples comparing(n ≥ 3) | 1. High quantification variation 2. Time consuming in sample processing & measuring 3. Limited sample multiplexing 4. Identify a proper spike-in standard based on target proteins and certain sample types. |
Triple-SILAC | Mixture of three different labeled cell lines | 1. Time saving 2. Low quantification variation 3.No need for SILAC standard | 1. Not applicable to tissues 2. More complexity of sample and MS spectra 3. Comparisons for only three samples |
