Figure 6 | Scientific Reports

Figure 6

From: Conserved RNA binding activity of a Yin-Yang 1 homologue in the ova of the purple sea urchin Strongylocentrotus purpuratus

Figure 6

Isolation of Messenger Ribonucleoprotein Particles from S. purpuratus ova. Lysates of S. purpuratus ova were either untreated (−RNase) or treated with RNase A/T1 (+RNase) and applied to oligo-dT cellulose columns and the bound proteins analyzed by SDS-PAGE and visualized by staining with Coomassie brilliant blue (upper panel) or subjected to Western blotting with anti-Xenopus YY1 antibody (lower panel). Fractions are indicated above the panel, L, load-on; U, unbound fraction; B, bound fraction. Positions of molecular mass markers and the YY1 band found upon Western blotting are indicated to the left of the panel.

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