Figure 6 | Scientific Reports

Figure 6

From: The anti-cancer activity of an andrographolide analogue functions through a GSK-3β-independent Wnt/β-catenin signaling pathway in colorectal cancer cells

Figure 6

Inhibition of the expression of Wnt/β-catenin proteins by RS-PP-050. (A) Immunoblot representing the decrease in protein expression of the active form of β-catenin in HT-29 cells after treatment with RS-PP-050 for 24 h. β-actin serves as a loading control. (B) Bar graph represents the normalized band intensities of the active forms of β-catenin to total β-catenin. Data are means ± S.E.M compared with the vehicle control (n = 3) (*P < 0.05, **P < 0.01). (C) Immunoblot representing the inhibition in protein expressions of active form of β-catenin in nuclear extracts of HT-29 cells after treatment with RS-PP-050 (10 μM) for 24 h. α-Tubulin, and lamin A/C serve as a cytosolic and a nuclear marker, respectively. For the cropped blots in Fig. 6A,C, protein samples were run under same conditional treatments and processed in parallel. Full-length blots are presented in Supplementary Fig. S2.

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