Figure 3
From: Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease
Proteomic profiling of serine proteases released by the human colonic mucosa. (A) Representative ABP proteomic profile, showing the differential repertoire of ABP-labelled serine proteases secreted from control or IBD colonic tissue samples along with the positive trypsin control (20 mU of trypsin). The red arrowheads point to bands corresponding to active proteases, as verified by the inhibitory effects of pre-treatment of the samples with AEBSF (4 mM). (B) Clustering of ABP-labelled serine proteases according to size (kDa). (C) Graphic representation of protease size clusters along with their activity index determined by the impact of enzyme inhibition (−/+AEBSF). The percentage of AEBSF-inhibited bands per patient is represented by the pie graphs. The empty circles represent patients wherein bands within the cluster were not detected (negative), a 0 value was given to these samples as per their activity index. Activity index data were analysed by Kruskal-Wallis followed by the multi comparison test of Dunn. *P < 0.05, **P < 0.01, vs. control; #P < 0.05 vs. CD.
