Figure 2
From: Polycomb Repressive Complex 2 attenuates the very high expression of the Arabidopsis gene NRT2.1
Reduction of H3K27me3 in clf-29 increases the expression of NRT2.1 in a context of very high expression. (A) Relative expression of NRT2.1 by qRT-PCR in roots of 7-days old of WT, clf-29, and swn-3 plants grown under high nitrogen (10 mM NH4NO3) or low nitrate (0.3 mM NO3−) conditions. Quantification by qRT-PCR is shown as the percentage of ACT2 transcript levels. (B) Relative GUS expression by qRT-PCR in roots of 7-days old ProNRT2.1:GUS WT and clf-29 plants grown under low nitrate (0.3 mM NO3−) condition. Quantification by qRT-PCR is shown as the percentage of ACT2 transcript levels. Error bars represent standard errors of the mean based on 3 biological replicates. Statistical significance was computed using a two-tailed Student’s t-test. Significance cutoff: *p < 0.05, **p < 0.01, ***p < 0.001.
