Figure 3
From: Expression and Regulation of Deubiquitinase-Resistant, Unanchored Ubiquitin Chains in Drosophila
Expression of Ub6-Stop everywhere or in specific tissues is not lethal to Drosophila. (A) Summary of the effects of expression of Ub6-Stop throughout fly development. Driver was sqh-Gal4. Control: sqh-Gal4 on the genetic background used to generate the Ub6 flies. We monitored daily lethality at larval, pupal and pharate adult stages among groups and did not notice differences in their development. Little to no developmental lethality occurred in all crosses. We monitored at least 10 independent crosses, all at 25 °C. (B,C) Percent longevity of adult flies not expressing or expressing Ub6-Stop at the indicated temperatures. P values were calculated using log-rank (Mantel-Cox) tests. (D) Western blots showing the expression levels of Ub6-Stop in the indicated tissues. We used one day old, whole adult flies for all lysates. Results are representative of experiments conducted independently four or more times, with similar results. Asterisk: non-specific band. (E) Summary of lethality during development when Ub6-Stop was expressed in specific tissues, as in (A). We monitored lethality at larval, pupal and pharate adult stages. We did not notice differences in their development and little to no developmental lethality occurred in all crosses. We monitored at least 10 independent crosses, all at 25 °C. (F) Percent longevity of adult flies not expressing or expressing Ub6-Stop in the indicated fly tissues. Controls consisted of the respective drivers on the genetic background utilized to generate Ub6 flies, but without the transgene. P values were calculated using log-rank (Mantel-Cox) tests. In all panels, flies were heterozygous for driver and Ub6.
