Figure 5
From: Expression and Regulation of Deubiquitinase-Resistant, Unanchored Ubiquitin Chains in Drosophila
Ub6-Stop interacts with the proteasome, VCP and p47 in Drosophila. (A–C) Western blots of co-IPs from whole fly heads expressing Ub6-Stop in fly eyes. Flies were heterozygous for GMR-Gal4 and HA-tagged Ub6-Stop. Asterisks: non-specific bands in lanes not expressing HA-Ub6-Stop. Results are representative of experiments conducted independently at least three times, with similar results. In each panel, “Ctrl” signifies anti-HA-bead-bound antibody that was incubated with fly lysates that do not express any HA-tagged Ub6-Stop. For quantified blots in panel (C), we loaded gels to achieve comparable levels of Ub6-Stop in the IP lanes. The entire HA-Ub6 smear was quantified. Shown in histograms are means −/+ SD. P values are from two-tailed Student’s t-tests.
