Figure 9
Characterization of SDS scaffolds repopulated with MuSCs and FBs. (a) Immunofluorescence analysis of scaffold cross-sections seeded with MuSCs-FBs and cultured for one week by using Pax7 (magenta) and ki67 (green), or MyoG (red) and laminin (green), or Tcf4 (magenta) and ki67 (green). Nuclei were stained with Hoechst (blue). Scale bar: 100 μm. (b) Percentage of Pax7-positive and negative cells in co-cultured scaffolds after one week. (c) SEM analysis showing cell-ECM interaction in SDS scaffolds seeded with MuSCs-FBs and cultured for one week. The insert show a single cell spreading its projections to the ECM. Scale bar: 20 μm left panel; 10 μm middle and right panel. (d) Immunofluorescence analysis of scaffold cross-sections seeded with MuSCs-FBs and cultured for two weeks by laminin (red) and eMyHC (green). Nuclei were stained with Hoechst (blue). Scale bar: 50 μm. (e) Immunofluorescence analysis of scaffold cross-sections seeded with MuSCs-FBs and cultured for two weeks by Pax7 (red) and ki67 (green). Nuclei were stained with Hoechst (blue). Scale bar: 50 μm.
