Table 1 Summary of the three decellularisation protocols.
LatB (1 cycle) | DET (3 cycles) | SDS (1 cycle) | ||||||
|---|---|---|---|---|---|---|---|---|
Time (h) | T (°C) | Reagent | Time (h) | T (°C) | Reagent | Time (h) | T (°C) | Reagent |
2 | 37 | 50 nM Latrunculin B in high glucose DMEM | ON | 4 | de-ionized water | 72 | RT | 0.25% SDS |
½ | RT | wash with PBS | 4 | RT | 4% sodium deoxycholate | 48 | RT | wash with de-ionized water |
2 | RT | 0.6 M potassium chloride | ½ | RT | wash with PBS | |||
2 | RT | 1.0 M potassium iodide | 3 | RT | 34 kU/ml DNase in 1 M NaCl | |||
ON | RT | de-ionized water | 48 | RT | de-ionized water | |||
2 | RT | 0.6 M potassium chloride | ||||||
2 | RT | 1.0 M potassium iodide | ||||||
2 | RT | 34 kU/ml DNase in 1 M NaCl | ||||||
ON | RT | de-ionized water wash | ||||||
