Table 3 Process suitability and matrix influence for PMX53 and PMX205 determination.
Spiked conc. plasma (ng/ml) & tissue (ng/g) | Process efficiency | Plasma | Brain | Spinal cord | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Recovery | Extraction efficiency | Matrix effect | Recovery | Extraction efficiency | Matrix effect | Recovery | Extraction efficiency | Matrix effect | |||
(mean ± SD%) | (mean ± SD%) | (mean ± SD%) | (mean ± SD%) | (mean ± SD%) | (mean ± SD%) | (mean ± SD%) | (mean ± SD%) | (mean ± SD%) | (mean ± SD%) | ||
PMX53 | LQC (6.25) | 98.1 ± 2.4 | 95.9 ± 0.4 | 95.3 ± 2.3 | 0.6 ± 0.5 | 97.2 ± 1.4 | 96.3 ± 3.3 | −2.8 ± 4.8 | 96.3 ± 1.4 | 97.1 ± 1.4 | 2.2 ± 0.1 |
MQC (25) | 94.2 ± 2.4 | 94.7 ± 2.3 | 97.2 ± 2.3 | 2.6 ± 0.5 | 99.1 ± 3.4 | 98.2 ± 3.9 | −0.85 ± 4.9 | 95.1 ± 1.4 | 99.1 ± 1.5 | 4.24 ± 1 | |
HQC (200) | 98.2 ± 0.6 | 99.1 ± 1.9 | 98.1 ± 1.9 | −0.94 ± 0.8 | 85.2 ± 0.4 | 84.6 ± 0.4 | −0.67 ± 0.7 | 99.1 ± 1.5 | 98.3 ± 1.5 | −0.7 ± 1.1 | |
PMX205 | LQC (6.25) | 94.1 ± 0.7 | 92.5 ± 0.7 | 95.1 ± 0.8 | 2.4 ± 0.2 | 85.9 ± 1.1 | 87.4 ± 0.9 | −2.0 ± 3.2 | 93.2 ± 6.2 | 95.4 ± 1.7 | 3.6 ± 0.5 |
MQC (25) | 93.4 ± 1.1 | 93.9 ± 2.2 | 96.5 ± 2.2 | 2.6 ± 0.5 | 87.1 ± 1.7 | 86.2 ± 1.6 | −0.8 ± 4.5 | 94.1 ± 5.5 | 98.1 ± 5.8 | 4.2 ± 0.9 | |
HQC (200) | 96.1 ± 0.9 | 90.6 ± 1.2 | 87.5 ± 1.2 | −3.5 ± 3.7 | 97.1 ± 5.1 | 99.5 ± 5.2 | 3.5 ± 1.2 | 93.8 ± 4.1 | 90.4 ± 3.9 | 3.6 ± 3.7 | |
