Table 4 Stability studies for PMX53 and PMX205.

From: Development and validation of a LC-MS/MS assay for pharmacokinetic studies of complement C5a receptor antagonists PMX53 and PMX205 in mice

 

Storage conditions

Spiked conc. plasma (ng/ml) & tissue (ng/g)

Plasma (n = 6)

Brain (n = 6)

Spinal cord (n = 6)

Measured conc. (ng/ml) (mean ± SD)

Precision (RSD, %)

Accuracy (RE, %)

Measured conc. (ng/g) (mean ± SD)

Precision (RSD, %)

Accuracy (RE, %)

Measured conc. (ng/g) (mean ± SD)

Precision (RSD, %)

Accuracy (RE, %)

PMX53

Short term (4 h at room temperature)

LQC (6.25)

6.24 ± 0.34

5.48

−0.20

6.28 ± 0.97

15.46

−0.06

6.56 ± 0.32

4.94

4.72

MQC (25)

24.81 ± 0.56

2.28

−0.78

24.9 ± 0.25

0.99

−0.46

24.9 ± 0.62

2.47

−0.49

HQC (200)

199.13 ± 2.71

1.36

−0.44

199.3 ± 3.6

1.81

−0.34

199.2 ± 3.1

1.57

−0.41

Freeze – thaw stability (3 cycles)

LQC (6.25)

6.24 ± 0.56

8.98

−0.18

6.36 ± 0.18

2.76

1.75

6.49 ± 0.86

13.20

3.64

MQC (25)

24.9 ± 0.31

1.25

−0.41

24.8 ± 0.63

2.54

−0.74

24.9 ± 0.16

0.63

−0.12

HQC (200)

199.97 ± 4.38

2.19

−0.01

199.8 ± 3.1

1.52

−0.42

199.7 ± 3.9

1.96

−0.16

Long term (−20 °C for 12 months)

LQC (6.25)

6.22 ± 0.88

14.10

−0.46

6.40 ± 0.45

7.01

2.32

6.33 ± 0.19

2.94

1.27

MQC (25)

24.88 ± 0.55

2.21

−0.50

24.9 ± 0.33

1.32

−0.24

24.9 ± 0.64

2.58

−0.20

HQC (200)

199 ± 2.83

1.42

−0.50

200.7 ± 4.5

2.25

0.34

199.9 ± 3.5

1.50

−0.07

PMX205

Short term (4 h at room temperature)

LQC (6.25)

5.91 ± 0.24

4.05

−5.80

6.18 ± 1.22

19.71

−1.18

5.99 ± 0.27

4.58

−4.34

MQC (25)

24.97 ± 1.28

5.14

−0.12

24.75 ± 1.71

6.84

0.56

25.07 ± 2.01

8.02

0.29

HQC (200)

197.6 ± 2.16

1.10

−1.19

198.9 ± 7.74

3.87

0.84

200.1 ± 2.57

1.28

0.45

Freeze – thaw stability (3 cycles)

LQC (6.25)

6.18 ± 0.81

13.16

−1.18

5.84 ± 0.20

3.34

−7.01

6.17 ± 1.36

22.07

−1.36

MQC (25)

24.76 ± 1.55

6.26

−0.95

24.73 ± 1.27

5.15

−1.10

24.87 ± 1.88

7.56

−0.51

HQC (200)

198.9 ± 2.9

1.46

−0.55

197.3 ± 2.24

1.13

−1.36

201.9 ± 6.86

3.40

0.95

Long term (−20 °C for 12 months)

LQC (6.25)

6.05 ± 0.68

10.90

−0.01

5.86 ± 0.30

5.09

−6.59

5.89 ± 0.19

3.25

−6.17

MQC (25)

24.8 ± 1.81

7.23

−0.01

24.32 ± 1.24

5.09

−2.79

24.82 ± 1.45

5.84

−0.72

HQC (200)

199.9 ± 3.24

1.62

−0.01

199.7 ± 2.30

1.15

−0.13

197.4 ± 2.57

1.30

−1.31

  1. Determination of PMX53 and PMX205 stability in different matrixes under different storage conditions.
  2. RSD: relative standard deviation; RE: relative error.
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