Figure 5 | Scientific Reports

Figure 5

From: Identification on mitogen-activated protein kinase signaling cascades by integrating protein interaction with transcriptional profiling analysis in cotton

Figure 5

Comparative yeast two-hybrid (Y2H) interaction analyses of MAPKKKs with MKKs. Protein interactions between MAPKKKs and MKKs were shown. Yeasts harboring the indicated plasmid combinations were grown on selective medium SD/-Ade/-His/-Leu/-Trp, positive interactions was examined by addition of Aureobasidin A and X-α-gal. Positive (pGBKT7-53 + pGADT7-rec) and negative (pGBKT7-Lam + pGADT7-rec) as controls. Three independent co-transformation experiments showed the similar results.

Back to article page