Figure 1 | Scientific Reports

Figure 1

From: Correlative atomic force microscopy quantitative imaging-laser scanning confocal microscopy quantifies the impact of stressors on live cells in real-time

Figure 1

AFM-QI-LSCM schematic illustration showing simultaneous data collection of multiplexed data. A live biological sample firmly immobilised to a substrate is mounted on a petridish. The inverted LSCM objective is focused onto the sample from below with the AFM cantilever and tip imaging from above. AFM-QI provides information on sample surface topography, (visco) elasticity and adhesion while LSCM provides localization of multiple fluorescently tagged molecules within the sample. Simultaneous AFM-QI-LSCM imaging provides high content data on live, actively dividing biological samples in an enriched aqueous environment.

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