Figure 1 | Scientific Reports

Figure 1

From: Evolutionary adaptation of an RNA bacteriophage to the simultaneous increase in the within-host and extracellular temperatures

Figure 1The alternative text for this image may have been generated using AI.

Evolutionary lineages of bacteriophage Qβ. Evolved populations Qβ(wt-p20)TR-1, Qβ(wt)TR-1, TR-2, and TR-3; and Qβ(A1956G)TR-4 were obtained upon propagation of the corresponding ancestral populations [Qβ(wt-p20), Qβ(wt), or Qβ(A1956G)] through the indicated transmission regimes (TR). TR-1 consisted of 10 cycles in which 10 min 60 °C heat shocks alternated with replication at 37 °C. TR-2 corresponded to 15 cycles of 10 min 60 °C heat shocks alternated with replication at an increased temperature that ranged from 39 °C to 43 °C. TR-3 consisted of 15 cycles in which 60 °C heat shocks (from 2.5 min to 10 min) alternated with replication at 43 °C. TR-4 was used only for population Qβ(A1956G), and consisted in its propagation through 15 transfers at 43 °C in the absence of heat shocks. All lineages were duplicated. Graphics at the bottom of the figure represent the pattern of change either in the duration of the heat shock or the replication temperature for each particular transmission regime.

Back to article page