Figure 4 | Scientific Reports

Figure 4

From: Homoharringtonine induced immune alteration for an Efficient Anti-tumor Response in Mouse Models of Non-small Cell Lung Adenocarcinoma Expressing Kras Mutation

Figure 4

HHT induced anti-tumor effects and alteration of immune cells in a KrasG12C-driven spontaneous mouse lung tumor model. (A) Left panel, India ink staining of lung tumors from the mice treated with and without HHT. Right panel, showing the number of lung tumor nodules. (B) Upper panel, showing cytokine expression levels in culture media of splenocytes from KrasG12C bi-transgenic mice treated with HHT, as detected by a cytokine antibody array. Lower panel, showing an array map. (C) Cytokine expression levels normalized to those of a positive control. (D) IL-12 expression levels of splenocytes isolated from KrasG12C-expressing tumor-bearing mice were measured using an ELISA. The columns and bars represent mean values ± SEM (n = 3 per group). (E) FACS analysis of B220+ CD86+ B cells and (F) B220+ CD69+ B cells in the spleen. The bar graph represents the average ± SEM (n = 3 mice per group). *p < 0.05, **p < 0.005, and ***p < 0.001. ns, no statistical difference.

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