Figure 3

Viability of M. smegmatis strains in the presence or absence of Ace. (A) Fluorescence microscopic images of live (green) and dead (red) bacteria are shown. AMI-mdp1 (MDP1) and AMI-NTD (NTD) cultured in the presence or absence of 0.2% Ace were stained with SYTO 9 (green) and propidium iodide (PI, red) using a Live/Dead BacLight Bacterial viability kit and examined by fluorescence microscopy. Representative cell images in each group are shown. Scale bars, 10 µm. (B) Using microscopic images detected in A, percentages of the live and dead cells (white and gray, respectively) were determined in five views (more than 150 cells in each view). Data from a single experiment is presented as mean ± SD of the results of five views. The result shown is representative of at least three experiments.