Table 3 Summary of imaging subjects with acute and chronic OM along with clinical history, OCT thickness values, corresponding nsOCT values, and additional notes.

Subject	Patient History	TM thickness (µm)	nsOCT value (nm)	Notes
Acute TM
A1	Normal	161	299
A2	Normal	173	297
A3	URI	143	299
A4	History of OM	172	299
A5	Normal	165	297
A6	Little sclerosis	171	300
A7	Normal	194	298
A8	URI	202	299
A9	Normal	160	296
A10	Normal	176	296
Chronic TM
C1	History of OM and tubes	193	299	Biofilm only
C2	Thickening of ear drums bilaterally	211	297	Effusion
C3	Effusion	207	298
C4	History of OM and tubes	199	294	Biofilm only
C5	OME	292	297
C6	OME	317	298
C7	OME	281	297
C8	Normal	196	299	Biofilm only
C9	OME	208	298
C10	OME	208	300
C11	OME	206	299	Effusion and biofilm
C12	OME	202	298
C13	URI	203	298
C14	OME	211	298
C15	URI	223	297
C16	Normal	238	298	Effusion
C17	Normal	204	299	Effusion and biofilm
C18	URI	240	300
C19	History of OM	245	300
C20	Normal	227	299	Biofilm only
C21	Normal	172	297	Effusion and biofilm
C22	OME	216	298
C23	Normal	207	299	Effusion
C24	OME	266	299
C25	OME	211	296
C26	URI	282	298
C27	URI with history of OM	224	297
C28	OME	205	297
Abnormal nsOCT
AB1	Normal	129	297
AB2	Normal	110	300
AB3	Normal	137	299	Viral infection
AB4	Normal	142	296
AB5	Normal	140	299
AB6	Normal	129	299
AB7	Normal	131	299	Right ear normal, but effusion on left ear
AB8	Normal	131	296	Viral infection
AB9	Normal	107	295	Viral infection
AB10	Normal	140	300
AB11	Normal	115	298
AB12	Normal	131	299	Viral Infection

OM = otitis media; TM = tympanic membrane; URI = upper respiratory infection; OME = otitis media with effusion; MEE = middle ear effusion; ASOM = acute suppurative otitis media.

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