Figure 4 | Scientific Reports

Figure 4

From: Use of Hemagglutinin Stem Probes Demonstrate Prevalence of Broadly Reactive Group 1 Influenza Antibodies in Human Sera

Figure 4

Stem-epitope dependent neutralization of H1N1 and H5N1 pseudoviruses. (A) Neutralization breadth of cross-reactive sera. Sera samples that bind to 4 or more HA SS probes were assayed for stem dependent neutralization of pseudotyped lentiviruses, H1 NC 99 and H5 IND 05, by neutralization competition assay. Samples with neutralization activity that is competed by WT but not Δstem HA is considered positive for stem directed nAb. The percentage of samples which neutralize H1 NC 99, H5 IND 05, both, or neither via the conserved stem epitope is shown. (B) Correspondence of sera neutralization activity to antibody titers. H1N1 (left) and H5N1 (right) neutralizing and non-neutralizing samples corresponding to respective ELISA endpoint titers against HA SS probes. Error bars (red) correspond to standard Mean plus SD.

Back to article page