Figure 2

Maximum clade credibility chronogram from 1,000 posterior trees generated using Beast. Dataset comprised 444 unique haplotypes (from total 1,122) of serrasalmid COI sequences (621 aligned base pairs). Bayesian posterior probabilities above 0.95 are shown as dark nodes. Point estimate species delimitations (total 118 unique delimitations) are shown by method as coloured boxes; due the large number of unique colours, some may appear similar. Rheophilic species are highlighted in bold blue font. Tree was presented using the ggtree_1.6.11 package97.