Table 1 Sequences of all short nucleotide substrates and their 5′- and 3′-modification by either Dyomics 681 (red) or 781 (green) that were used in the RNase activity assays.
From: Homodimerisation-independent cleavage of dsRNA by a pestiviral nicking endoribonuclease
Name | Sequence | 3′ and 5′ Modification |
|---|---|---|
ssRNA+ | 5′-GCC CGU CUG UUG UGU GAC UCG CUC GUC UGC-3′ | Dyomics 681 |
ssRNA− | 5′-GCA GAC GAG CGA GUC ACA CAA CAG ACG GGC-3′ | Dyomics 781 |
poly(A) | 5′-AAA AAA AAA AAA AAA AAA AA-3′ | Dyomics 681 |
poly(U) | 5′-UUU UUU UUU UUU UUU UUU UU-3′ | Dyomics 781 |
poly(C) | 5′-CCC CCC CCC CCC CCC-3′ | Dyomics 681 |
poly(G) | 5′-GGG GGG GGG GGG GGG-3′ | Dyomics 781 |
metRNA (−) | 5′-GCA GAC GAG CGA GUC ACA CAA CAG ACG GGC-3′ | Dyomics 781 |
ssDNA (−) | 5′-GCA GAC GAG CGA GTC ACA CAA CAG ACG GGC-3′ | Dyomics 781 |
