Figure 2 | Scientific Reports

Figure 2

From: eIF4A inhibition prevents the onset of cytokine-induced muscle wasting by blocking the STAT3 and iNOS pathways

Figure 2

Hippuristanol (Hipp) prevents IT-mediated activation of the pro-cachectic iNOS/NO pathway. (A) Western blot analysis of iNOS protein levels in myotubes treated with or without IT in the presence or absence of Hipp (100 and 200 nM) was performed using an anti-iNOS antibody. Tubulin was included as a loading control. (B) The aforementioned Western blot for iNOS was quantified and standardized to Tubulin levels. Data are shown relative to IT-induced iNOS protein levels and plotted ± s.e.m **P < 0.01, ***P < 0.001 Student’s T-test (n = 3). (C) NO production in myotubes treated as described in (A) was determined using the Griess assay and plotted ± s.e.m ***P < 0.001 Student’s T-test (n = 4).

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