Figure 4
Hippuristanol (Hipp) inhibits STAT3 protein, but not STAT3 mRNA levels, in IT-treated myotubes. (A) Western Blot analysis of myotubes treated with or without IT in the presence or absence of Hipp using antibodies against phosphotyrosine705 STAT3, STAT3 and Tubulin (loading control). (B) phosphotyrosine705 STAT3 and (C) STAT3 levels shown in the Western blots in (A) were quantified, standardized to Tubulin and normalized relative to IT-treated myotubes. Values were plotted ± s.e.m *p < 0.05, **p < 0.01 Student’s T-test (n = 3). (D) RT-qPCR analysis on RNA derived from myotubes treated as described in (A) using primers against STAT3, standardized to the housekeeping gene RPL32 and normalized relative to IT-treated myotubes. Values were plotted ± the s.e.m. N.S. Student’s T-Test (n = 3).
