Figure 2

Antigenicity of polymorphic DBL-TH antigens at acute and convalescence phase of P. vivax infection. The total IgG antibody response against a panel of DBL-TH variant antigens and DBPII-Sal I was measured by conventional ELISA method. Human plasma from acutely infected P. vivax patients (AC, n = 40), 3-months post-infection (3 m, n = 36), 9 months post-infection (9 m, n = 29,) and 12-months post-infection (12 m, n = 27) were tested for antibody detection specific for DBP antigens, DBPII- Sal I, DBL-TH2, -TH4, -TH5, -TH6, and -TH9. (a) Box plot with 10^th- and -90^th-percentile values represented IgG response against antigens tested at each time point. The dashed line represents the cut-off value for each antibody response to a relative antigen equal to the mean plus 2 SD of the OD of healthy control. (b) The intensity of responder was classified into NR (white bar) refers to negative responders (<cut-off); LR (grey bar) refers to low responders (>cut-off but <cut-off + 2 SD of the OD of HC); HR refers to high responders (≥cut-off + 2 SD of the OD of HC).