Figure 1

Screening and identification of FRα binding peptides. A Ph.D.-12 phage library was used to screen FRα binding phages with four rounds of biopanning. (A) The enrichment of FRα binding phages were evaluated by phage recovery yields of each round selection. (B) Polyclonal phage ELISA using elutes after each round selection. (C) 94 phage clones were randomLy picked from the third round selection, and their binding affinities for FRα were analyzed individually by phage ELISA.