Figure 6
Whereas Tgfb1 treatment causes divergent shifts in steady-state levels of a subset of PrP interactors, PrP-depletion in the same paradigm only affected Ncam1 protein levels. (a) Schematic depicting nature of global proteome datasets mined for this study. A more complete presentation of these datasets had been published before17. (b) Comparison of steady-state protein levels of a subset of PrP interactors before and after Tgfb1 induced mesenchymal differentiation. The steady-state levels of proteins with predominant localization in the ER/Golgi compartments did not change upon two-day exposure of NMuMG cells to Tgfb1 (bottom half of table). However, proteins PrP is expected to be surrounded by at the plasma membrane underwent divergent changes in their steady-state levels. The stable PrP knockdown did not affect the protein levels of PrP candidate interactors, except for Ncam1, whose levels were diminished in PrP-deficient cells. Please see Supplementary Table S2 for a more complete presentation of underlying quantitations.
