Figure 2 | Scientific Reports

Figure 2

From: Environmental responsiveness of tubulin glutamylation in sensory cilia is regulated by the p38 MAPK pathway

Figure 2

Tubulin glutamylation in mouse retina is modulated by the light exposure. (a) Schematic diagram of a mammalian photoreceptor cell (rod). The region was divided into three areas: outer segment, transition zone, and inner segment. (b) Result of immunohistochemical analysis of mouse retina using anti-polyglutamate polyclonal antibody (top, red), α-tubulin (second row, green), merged (third row), and DIC (bottom). Nuclei are shown with DAPI staining (blue). Left column: dark adaptation. Right column: light adaptation. Polyglutamate signals can be observed in connective cilia (red arrow). Tubulin signals were observed in the transition zone and inner segment. Tubulin glutamylation co-localised with tubulin in the transition zone. OS: outer segment, TZ: transition zone, IS: inner segment, ONL: outer nuclear layer. (c) Quantitative analysis of five independent mice. All mice have a significant reduction of tubulin glutamylation (p < 0.05). Scale bars = 20 μm.

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