Figure 7
Spp1 (osteopontin) mRNA and Spp1 protein expression were reduced in Tg6F fed mice. Total RNA and protein were isolated from the lungs of the BALB/c mice described in Fig. 1. (a) Spp1 mRNA expression was quantified by RT-qPCR (left side of panel) and protein lysates from lungs were analyzed by immunoblotting for Spp1 (right side of panel) as described in Materials and Methods. Each lane in the immunoblot is from a separate mouse. (b) Spp1 mRNA expression was reduced in the intestines of Tg6F fed mice. Total RNA was isolated from the jejunum of the BALB/c mice described in Fig. 1. Spp1 mRNA expression was quantified by RT-qPCR as described in Materials and Methods. The data shown are Mean ± SEM. (c) Tg6F decreased Spp1 levels in the plasma as measured by ELISA as described in Materials and Methods. The data shown are Mean ± SEM. (d) Cholesterol 25-hydroxylase (CH25H) protein expression in the villi of the jejunum was quantified by immunohistochemistry as described in Materials and Methods. The results shown are Mean ± SEM. (e) 25-hydroxycholesterol (25-OHC) levels in jejunum were determined by LC-MS/MS as described in Materials and Methods. The results shown are Mean ± SEM.
