Figure 5

Comparison of ANS binding of fLCA (closed diamond) and tLCA (open square). A 70:1 ANS/protein ratio was used; excitation and emission wavelength of ANS was 370 nm and 488 nm, respectively. Error bars represent the standard deviation of at least 3 samples. The small bump at 37 °C indicated with an arrow was observed previously in fLCA and characterized as the PRIME conformation5.