Table 1 Summary of activity of fLCA and tLCA with peptide and full-length substrates.

From: A novel role of C-terminus in introducing a functionally flexible structure critical for the biological activity of botulinum neurotoxin

LCA

Temp. (°C)

Substrate

% cleavage

% activity compared to fLCA at 37 °C

fLCA

25

peptide

3.9% ± 0.3%

33%

fLCA

37

peptide

12% ± 1.8%

100%

tLCA

25

peptide

1.4% ± 0.1%

12%

tLCA

37

peptide

4.5% ± 0.3%

38%

fLCA

37

full-length

38.9% ± 1.1%

100%

tLCA

37

full-length

19.6% ± 1.4%

50%

  1. Shown are the % cleavage of substrates after 30 min (peptide) or 48 hrs (full-length in M17 neuroblastoma cells), each performed in at least triplicate.
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