Figure 4

Intermittent fasting induced a decrease in central area exploration in HT animals in the open field test. (a) Curve of distance travelled, two-way ANOVA followed by Holm-Sidak’s post hoc test, P > 0.05, n = 8 (C/HT, IF/HT), 11 (C/WT), and 12 (IF/WT); (b) distance travelled in first 5 min, two-way ANOVA followed by Holm-Sidak’s post hoc test, P > 0.05, n = 7 (C/HT), 8 (IF/HT) and 11 (C/WT, IF/WT); (c) mean speed in first 5 min, two-way ANOVA followed by Holm-Sidak’s post hoc test, P > 0.05, n = 7 (C/HT), 8 (IF/HT) and 11 (C/WT, IF/WT); (d) total time of freezing behavior in first 5 min, two-way ANOVA followed by Holm-Sidak’s post hoc test, P ≤ 0.05 for the treatment factor, n = 7 (C/HT), 8 (IF/HT) and 11 (C/WT, IF/WT); (e) curves for time in central and peripheral areas; (f) time in central area in first 5 min, two-way ANOVA followed by Holm-Sidak’s post hoc test, ^*P ≤ 0.05, n = 7 (C/HT), 8 (IF/HT) and 11 (C/WT, IF/WT); (g) time in peripheral area in first 5 min, two-way ANOVA followed by Holm-Sidak’s post hoc test, ^*P ≤ 0.05, n = 7 (C/HT), 8 (IF/HT) and 11 (C/WT, IF/WT); (h) representative heat map plot for animal movement in the apparatus.