Table 1 Summary of western blotting statistical analyses.
Protein analyzed | ANOVA factor or Post-hoc test | Groups compared | F (DFn, DFd) | P value | Statistical significance? |
|---|---|---|---|---|---|
PTEN | Interaction | Treatment × Genotype | F (1, 32) = 0.4084 | 0.5273 | — |
Treatment | Control × IF | F (1, 32) = 0.7822 | 0.3831 | — | |
Genotype | WT × HT | F (1, 32) = 6.356 | 0.0169 | Yes | |
Holm-Sidak’s multiple comparisons test | C/WT × C/HT | — | 0.1525 | — | |
IF/WT × IF/HT | — | 0.5752 | — | ||
C/WT × IF/WT | — | 0.5877 | — | ||
C/HT × IF/HT | — | 0.8633 | — | ||
AKT | Interaction | Treatment × Genotype | F (1, 32) = 0.07327 | 0.7884 | — |
Treatment | Control × IF | F (1, 32) = 0.1903 | 0.6656 | — | |
Genotype | WT × HT | F (1, 32) = 0.4513 | 0.5065 | — | |
p-AKT T308/AKT | Interaction | Treatment × Genotype | F (1, 32) = 4.027 | 0.0533 | — |
Treatment | Control × IF | F (1, 32) = 1.024 | 0.3191 | — | |
Genotype | WT × HT | F (1, 32) = 4.288 | 0.0465 | Yes | |
Holm-Sidak’s multiple comparisons test | C/WT × C/HT | — | 0.0412 | Yes | |
IF/WT × IF/HT | — | 0.9642 | — | ||
C/WT × IF/WT | — | 0.8422 | — | ||
C/HT × IF/HT | — | 0.1707 | — | ||
p-AKT S473/AKT | Interaction | Treatment × Genotype | F (1, 32) = 0.07327 | 0.7884 | — |
Treatment | Control × IF | F (1, 32) = 0.1903 | 0.6656 | — | |
Genotype | WT × HT | F (1, 32) = 0.4513 | 0.5065 | — | |
S6 | Interaction | Treatment × Genotype | F (1, 32) = 0.02098 | 0.8857 | — |
Treatment | Control × IF | F (1, 32) = 0.4574 | 0.5037 | — | |
Genotype | WT × HT | F (1, 32) = 1.428 | 0.2409 | — | |
p-S6/S6 | Interaction | Treatment × Genotype | F (1, 32) = 0.9075 | 0.3479 | — |
Treatment | Control × IF | F (1, 32) = 0.7840 | 0.3825 | — | |
Genotype | WT × HT | F (1, 32) = 1.366 | 0.2512 | — | |
AMPA | Interaction | Treatment × Genotype | F (1, 32) = 1.087 | 0.3050 | — |
Treatment | Control × IF | F (1, 32) = 0.2580 | 0.6150 | — | |
Genotype | WT × HT | F (1, 32) = 0.7687 | 0.3872 | — | |
NR1 | Interaction | Treatment × Genotype | F (1, 32) = 0.8696 | 0.3580 | — |
Treatment | Control × IF | F (1, 32) = 1.877 | 0.1803 | — | |
Genotype | WT × HT | F (1, 32) = 1.871 | 0.1809 | — | |
NR2a | Interaction | Treatment × Genotype | F (1, 31) = 1. 367 | 0.2513 | — |
Treatment | Control × IF | F (1, 31) = 0.03016 | 0.8633 | — | |
Genotype | WT × HT | F (1, 31) = 1.380 | 0.249 | — | |
NR2b | Interaction | Treatment × Genotype | F (1,24) = 0.9843 | 0.3310 | — |
Treatment | Control × IF | F (1,24) = 0.2853 | 0.5981 | — | |
Genotype | WT × HT | F (1,24) = 1.885 | 0.1825 | — | |
PSD-95 | Interaction | Treatment × Genotype | F (1, 32) = 1.896 | 0.1781 | — |
Treatment | Control × IF | F (1, 32) = 0.7113 | 0.4053 | — | |
Genotype | WT × HT | F (1,32) = 1.054 | 0.3123 | — | |
Synaptophysin | Interaction | Treatment × Genotype | F (1, 31) = 0.5522 | 0.4630 | — |
Treatment | Control × IF | F (1, 31) = 1.313 | 0.2606 | — | |
Genotype | WT × HT | F (1, 31) = 0.08962 | 0.7667 | — |
