Figure 1 | Scientific Reports

Figure 1

From: Differential requirement for dimerization partner DP between E2F-dependent activation of tumor suppressor and growth-related genes

Figure 1

DP1 knockdown does not compromise E2F-induced ARF mRNA expression or activation of p53. (A) Expression of DP family members in human normal fibroblast WI-38 cells. RT-PCR analysis of DP family genes with 25 cycles of PCR using same amount of cDNA. *Non-specific bands. Full-length gel is presented in Supplementary Fig. S1. (B) Time frame of knockdown experiments. (C,D) RT-PCR analysis of mRNA induction by ectopic expression of E2F1 (C) or E1a (D) in WI-38 cells expressing DP1-shRNA. Full-length gels are presented in Supplementary Figs S2(C) and S3(D). (E,F) Immunoblot analysis of DP1 expression and induction of ARF and p53 by ectopic expression of E2F1 (E) or E1a (F) in WI-38 cells expressing DP1-shRNA. Full-length blots are presented in Supplementary Figs S4(E) and S5(F).

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