Figure 3 | Scientific Reports

Figure 3

From: Differential requirement for dimerization partner DP between E2F-dependent activation of tumor suppressor and growth-related genes

Figure 3

DP1 does not cooperate with deregulated E2F1 to bind to or activate ARF promoter. (A,B) RT-PCR analysis (A) and immunoblot analysis (B) in WI-38 cells infected with Ad-E2F1 (MOI 20) and/or Ad-DP1 (MOI 100). Full-length gels and blots are presented in Supplementary Figs S9(A) and S10(B). (C) Reporter analyses of CDC6 and ARF promoters in WI-38 cells expressing E2F1 and DP1. **P < 0.01. (D) Gel mobility shift assays using GST-E2F1 and GST-DP1. Probes were a DHFR promoter fragment containing typical E2F sites (left panel) and EREA (two middle panels). Western blot analysis of purified GST-E2F1 and GST-DP1 (right panel). Full-length blots are presented in Supplementary Fig. S11. (E) ChIP analysis of E2F target promoters in WI-38 cells infected with Ad-E2F1 (MOI 20) and/or Ad-DP1 (MOI 100). Full-length gels are presented in Supplementary Fig. S12.

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