Figure 2 | Scientific Reports

Figure 2

From: Mapping of Adenovirus of serotype 3 fibre interaction to desmoglein 2 revealed a novel ‘non-classical’ mechanism of viral receptor engagement

Figure 2

Mapping of EC domains required for fibre binding (A) Schematic representation of the different recombinant DSG2 constructs EC1-4, EC1-3 and EC2-3. (B) Gel filtration based co-elution experiments. Size exclusion chromatography analysis of Ad3K co-incubated with the different constructs demonstrates a shift of elution to smaller retention volumes in each case thus reflecting an increased molecular weight. This is an agreement with a complex formation between DSG2 and Ad3K. (C) 10 or 4–20% SDS-PAGE analysis of the peak fractions aligned to its corresponding chromatogram show the presence of both Ad3K and DSG2 construct (as indicated) in the shifted peak confirming the formation of a complex.

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