Figure 6

Stoichiometry analysis of the Ad3K + EC2-3 complex. (A) Molecular mass determination by SEC-MALLS. Overlay of SEC elution profiles of Ad3K (grey line) and Ad3K in complex with EC2-3 (black line). The lines in green or blue represent the average molecular weight of the major Ad3K and Ad3K + EC2-3 species calculated by using the light-scattering measurements (scale on the right y axis). (B) Analytical ultracentrifugation (AUC) analysis. Normalized c(s) distribution curves calculated from the raw data using SEDFIT are shown for Ad3K (in blue) and for Ad3K in complex with EC2-3 (in green). The resulting sedimentation coefficients are indicated for the main peaks.