Table 1 Glycan composition of the different N-glycosylations within cell-free synthesized EPO, identified by nano-liquid chromatography mass spectrometry based on combined information from collision-induced dissociation and electron transfer dissociation fragmentation.

From: Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin

residue

Peptide sequence

MWexp (Da)

Mwtheor (Da)

Glycan composition

N38

HCSLNdeamEN*ITV

1129.7

1129.52

Man7HexNAc2 Man6HexNAc2 Man5HexNAc2

N24

EAEN*ITTGCcarbAE

1193.5

1193.49

Man8HexNAc2 Man7HexNAc2

N83

LVN*SSQPWEPLQdeamL

1510.7

1510.77

Man8HexNAc2 Man7HexNAc2 Man6HexNAc2 Man5HexNAc2