Table 1 Glycan composition of the different N-glycosylations within cell-free synthesized EPO, identified by nano-liquid chromatography mass spectrometry based on combined information from collision-induced dissociation and electron transfer dissociation fragmentation.
From: Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin
residue | Peptide sequence | MWexp (Da) | Mwtheor (Da) | Glycan composition |
|---|---|---|---|---|
N38 | HCSLNdeamEN*ITV | 1129.7 | 1129.52 | Man7HexNAc2 Man6HexNAc2 Man5HexNAc2 |
N24 | EAEN*ITTGCcarbAE | 1193.5 | 1193.49 | Man8HexNAc2 Man7HexNAc2 |
N83 | LVN*SSQPWEPLQdeamL | 1510.7 | 1510.77 | Man8HexNAc2 Man7HexNAc2 Man6HexNAc2 Man5HexNAc2 |