Figure 2
Phenotypic and metabolic states of WT Mtb and BCG inside THP-1 macrophages. (A) CFU-based viability test of WT Mtb and BCG inside THP-1 over 9 days of incubation. *P < 0.001 by Student’s unpaired t-test. (B) Heatmap profile of THP-1 following either infection with WT Mtb or BCG at MOI 1.0 or treatment of 10 µg/mL LPS. Columns depict experimental conditions, starting with untreated control followed by WT Mtb, BCG infection, and LPS treatment. Rows indicate metabolites of the bacterial propionate pathway (methylcitrate cycle, MCC). Data are depicted on a log2 scale relative to untreated control for each experimental condition. (C) Fold changes of MCC intermediates relative to those in uninfected control. Change of itaconate was included as a control in THP-1 activation. *P < 0.001; and N.S, not significant by Student’s unpaired t-test.
