Figure 3
Intrinsic downregulation of methylisocitrate lyase activity causes toxic MCC intermediates in BCG. (A) The schematic describes the genes and intermediates in the bacterial methylcitrate cycle, MCC. (B,C) Expression levels of transcripts of prpC, prpD and icl1 in WT Mtb or BCG cultured under G/P carbons relative to their counterparts under G/D carbons are described (B) and their changes are demonstrated by fold change relative to the average of the first two genes (prpC and prpD). Asterisk indicates the relative level of BCG icl1 transcript as the lowest (C). (D) MCL-ABMP mediated time-course measurements of MCL substrate (2MC/2MI) and product (succinate) in the presence of either WT Mtb or BCG small metabolome extract (SME) under G/P carbons. All values are average of two independent experiments, each of which consisted of biological triplicates ± SD.
