Figure 5
From: Effect of HIV-1 Tat on the formation of the mitotic spindle by interaction with ribosomal protein S3
Ectopic expression of mCherry-RPS3mt rescues the mitotic defects. (A) Expression of mCherry-RPS3mt rescues mitotic defects in RPS3-knockdown cells. HeLa-H2B-GFP cells were transfected with the pmCherry or pmCherry-rps3mt plasmid 48 h after transduction of shRPS3-4 and incubated for a further 7 days in the presence of 2 µg/ml puromycin and 500 µg/ml geneticin. (B) mCherry-RPS3mt expression in RPS3-knockdown cells. A low level of mCherry-RPS3 is expressed in the cytosol of RPS3-knockdown cells. (C) mCherry-RPS3mt localizes in the mitotic spindle. mCherry-RPS3mt was concentrated around chromosomes and formed mitotic spindle-like threads, and chromosome assembly was not aberrant in metaphase.
