Figure 8
From: Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo
Hematopoietic cell-specific Postn deletion altered adipose tissue remodeling. (a) Representative H&E staining images of adipose tissue (Epi-fat) of BM transplanted mice. (b) Left, representative Masson’s trichrome staining images of adipose tissue (Epi-fat) of BM transplanted mice. Right, quantification of Masson’s trichrome-positive area (identical with interstitial fibrosis). n = 3 images per mouse, 5–10 mice each. ****p < 0.0001 by unpaired t-test. (c) Left, representative immunofluorescent staining of F4/80 (red) and perilipin (green). Right, the number of F4/80-positive cells in Epi-fat of BM transplanted mice. Scale bars, 100 μm. ****p < 0.0001 by unpaired t-test. (d) Collagen (Col1a1, Col3a1 and Col6a1) mRNA levels in Epi-fat from BM transplanted mice. n = 9–15, *p < 0.05 by unpaired t-test. (e) Pro-inflammatory (Emr1, Mcp1 and Tnf-α) mRNA levels in Epi-fat from BM transplanted mice. n = 9–15, *p < 0.05 by unpaired t-test.
