Table 1 Optimal MS/MS parameters for the detection of Glc-PE and Lac-PE in the MRM and PIS modes.
Mode | Amadori-PE | Species | Precursor ion (m/z) | Product ion (m/z) | DP (V) | EP (V) | CE (V) | CXP (V) |
|---|---|---|---|---|---|---|---|---|
MRM | Glc-PE | 34:1 | 902.6 | 326.1 | 120 | 10 | 45 | 18 |
36:1 | 930.6 | 326.1 | 102 | 10 | 53 | 18 | ||
36:2 | 928.6 | 326.1 | 102 | 10 | 53 | 18 | ||
Lac-PE | 34:1 | 1064.6 | 488.1 | 125 | 10 | 56 | 30 | |
36:1 | 1092.7 | 488.1 | 115 | 10 | 57 | 28 | ||
36:2 | 1090.6 | 488.1 | 115 | 10 | 57 | 28 | ||
PIS | Glc-PE | 326.1 | 90–156 | 10 | 45 | 18 | ||
Lac-PE | 488.1 | 115–166 | 10 | 56 | 28 |