Figure 4

Fluorescence imaging of endogenous Zn²⁺ in acute rat hippocampal slices. Slice incubated with BP & Fluozin-3 AM and imaged using 460–490 nm excitation and 520 nm emission respectively (a & b). Slice incubated with TSQ and imaged using 330–385 nm excitation and 420 nm emission (c). H marks the area of dentate Hilus, DG- Dentate gyrus, CA1, CA2 & CA3 – Cornu ammonis 1, 2 & 3, SL- Stratum lucidum, SP- Stratum pyramidale, GCL- Granular cell layer, ML- Molecular layer. 4 × Magnification, images stitched manually, scale bar = 300 µm.