Figure 7

Ratiometric fluorescence imaging of endogenous Zn²⁺ and its dynamics showing the translocation. Control slice with BP and stimulated epileptic slice with BP are shown in the 1^st and 2^nd rows respectively. (a,d) represents fluorescence images with emission collected at 510–550 nm and (b,e) represents that collected at 575–625 nm. Ratiometric images (I₆₂₀/I₅₇₀) generated from 1^st and 2^nd columns are shown in c and f. Magnification 1.25 × (control), 4 × (stimulated), scale bar = 200 µm. Ratio bar represents Zn²⁺ concentration from low (black) to high (red). Arrows in (d,e) indicates the intense fluorescence due to artefact which is cancelled out in (f).