Figure 5 | Scientific Reports

Figure 5

From: Confirmation of high-throughput screening data and novel mechanistic insights into VDR-xenobiotic interactions by orthogonal assays

Figure 5

Protein: protein interaction between VDR with RXRα, SRC-1 and NCoR in the presence of select agonists: (a) Recruitment of coactivator SRC-1 (SRC/p160 family) by VDR in the presence and absence of RXRα: (b) Recruitment of heterodimerization partner RXRα by VDR in the presence and absence of SRC-1 (c) Recruitment of corepressors NCoR by VDR in the presence and absence of RXRα. Assays were run in Cos7 cells and data expressed as mean ± SEM (n = 3). Data are normalized to VDR + empty pM vector. Only significant (at least p < 0.05) data points are expressed as a percentage of vitamin D3. EpSG5 represents empty pSG5 vector in which RXRα or SRC-1 is expressed.

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