Figure 4
Effect of oligomycin and rotenone on TMRM intensity. TMRM fluorescence intensity measured in cells following exposure to oligomycin (A) p < 0.05 Kruskal-Wallis test, or rotenone (B), p < 0.05–p < 0.001 Kruskal-Wallis test, normalised to basal for each line. TMRM fluorescence intensity measured in mitochondria following exposure to oligomycin (C), p < 0.01 Kruskal-Wallis test, or rotenone (D) p < 0.05–p < 0.0001 Kruskal-Wallis test normalised to basal for each line. Dots represent mean data for each image, all data normalised to basal for each cell line, basal mean represented by dotted line, error line and bars represent median ± interquartile range, n = 3 independent experiments.
