Figure 9
Assessment of cell viability in control and patient lines within mitochondrial function assay. Cell viability was analysed by 2 measures: (1) the number of live cells analysed, (2) the percentage of dead cells identified. Change in number of live cells (A) or percentage of dead cells (B) assessed following exposure to CCCP, oligomycin or rotenone, normalized to basal for each line for live cell change. No significant difference from basal for each condition, n = 3 individual experiments. Effect of H2O2 on the number of live cells (C) and percentage of dead cells (D), one-way ANOVA p < 0.005–0.001 for dead cells exposed to H2O2 compared to basal for each line, n = 2 independent experiments. Dots represent data for individual experiments, lines represent mean ± SEM.
