Figure 4
From: Enhanced quantification of metabolic activity for individual adipocytes by label-free FLIM
Mitochondria stress test. (a) Fluorescence intensity of the individual lifetime components of NADH for each step of the mitochondria stress test. (b) The effect of individual injections of oligomycin, FCCP, and rotenone/antimycin on the FLIM-derived MCR, optical free-to-bound ratio, and redox ratio as well as on OCR and ECAR measured by an extracellular flux analyzer. Mean ± SD of n = 40 FLIM images per group. Significant differences of treatment groups to the control by Student’s t-test with *p < 0.05, **p < 0.001.
