Figure 5
From: Enhanced quantification of metabolic activity for individual adipocytes by label-free FLIM
Glycolysis stress test. (a) Fluorescence intensity of the individual lifetime components of NADH for each step of the glycolysis stress test. (b) The effect of individual injections of glucose, oligomycin, and 2-DG on the FLIM-derived MCR, optical free-to-bound ratio, and redox ratio as well as on OCR and ECAR measured by an extracellular flux analyzer. Mean ± SD of n = 40 FLIM images per group. Significant differences of treatment groups to the control by Student’s t-test with *p < 0.05, **p < 0.001.
