Figure 6
From: Enhanced quantification of metabolic activity for individual adipocytes by label-free FLIM
Murine white inguinal fat after 1-hour treatment with epinephrine and forskolin compared to untreated cells. (a) Expression of UCP1 and housekeeping gene GAPDH. UCP1 expression is normalized to the expression of GAPDH. Full length western blots are shown in Supplementary Fig. 6. (b) Effect on the FLIM-derived free-to-bound ratio and MCR, OCR and ECAR measured by the extracellular flux analyzer, and the gene expression of browning marker UCP1 analyzed by western blotting. Mean ± SD of n = 20 FLIM images per group. Significant differences of treatment groups to the control by Student’s t-test with *p < 0.05, **p < 0.001.
